Teichoic Acid Phosphorylcholine Esterase

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چکیده

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Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce.

The pneumococcal phosphorylcholine esterase (Pce or CbpE) is a modular protein that hydrolyses the phosphorylcholine residues present in the teichoic and lipoteichoic acids of the pneumococcal cell wall. Pce has been crystallized using the hanging-drop vapour-diffusion method at 291 K. Diffraction-quality monoclinic crystals belong to space group C2, with unit-cell parameters a = 169.82, b = 57...

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Wall Teichoic Acid Glycosylation

The biosynthetic enzymes involved in wall teichoic acid biogenesis in Grampositive bacteria have been the subject of renewed investigation in recent years with the benefit of modem tools of biochemistry and genetics. Nevertheless, there have been only limited investigations into the enzymes that glycosylate wall teichoic acid. Decades-old experiments in the model Gram-positive bacterium, Bacill...

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Streptococcal Teichoic Acid

Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A, D, E, 0, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B, C, F, G, H, K, L, M, N, P, Q, R, and S did not give a positive reaction with group antisera. The group A material also reacted with anti-E serum...

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Teichoic acid synthesis in Bacillus stearothermophilus.

1. Particulate enzyme preparations obtained from Bacillus stearothermophilus B65 by digestion with lysozyme were shown to catalyse teichoic acid synthesis. With CDP-glycerol as sole substrate the preparations synthesized 1,3-poly(glycerol phosphate). It was characterized by alkaline hydrolysis, by glucosylation to the alkali-stable 2-glucosyl-1,3-poly(glycerol phosphate) with excess of UDP-gluc...

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Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization

UNLABELLED Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms that govern WTA-mediated nasal colonization have remained elusive. Here, we report that ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1974

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)42162-5